c33a cell lines  (ATCC)

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: MTT assay results for cytotoxicity of drug treatments. Drug dose–response curves after 48 h for HPV- C33a, HPV+ CaSki, and normal epithelial cell line CRL1790: ( A ) cisplatin, ( B ) EPZ6438, and ( C ) ZLD1039. Data is expressed as log of mean ± SD (n = 3). IC50 values, pinpointed on the graph, were calculated using GraphPad prism. ( D ) Table of mean IC50 value data (n = 3). The IC50 value for EPZ6438 could not be determined within the 0–80 µM range.

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: MTT Assay

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: Migration rate assessment following the scratch wound–healing assay. ( A ) Representative images from the in vitro scratch wound–healing assays demonstrating cell migration into the cell-free region (outlined by black lines) following 48 h treatment (scale = 100 µm). ( B ) Summary plots showing the migration rates by C33a and CaSki cells after treatment (Mean ± SD, n = 3).

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: Migration, Wound Healing Assay, In Vitro

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: Effect of EZH2 inhibitors on protein and mRNA expression of ZO-1 and E-cadherin on cervical cancer cells after 48 h treatment. ( A ) Immunofluorescence staining of ZO-1 and E-cadherin protein expression (pink) and nuclei (blue) under 400× microscope magnification. Scale = 50 µm. Mean fluorescence intensity of immunocytochemistry results were normalised to control cells (right) (n = 3). ( B ) Representative Western blots’ results of protein bands ZO-1 (250 kDa) and β-actin (42 kDa) for C33a and CaSki and E-cadherin (110 kDa) for CaSki are shown on the left. Bar charts on the right show the Western blot quantification of these protein expression levels following treatment. Values are expressed as a fold change of control (n = 3). ( C ) RT-qPCR analysis of relative ZO-1 (C33a and CaSki) and E-cadherin (CaSki) mRNA expression levels following 48 h treatment. Values are expressed as fold change of control values. Data is presented as mean ± SD (n = 3). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Bold asterisk above a single sample bar indicates the significant difference of that sample from the rest of the treatments.

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: Expressing, Immunofluorescence, Staining, Microscopy, Fluorescence, Immunocytochemistry, Control, Western Blot, Quantitative RT-PCR

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: Immunohistochemical staining results of EZH2, p53, ZO-1, β-catenin, and HPV16 E6 from CAM assay tissue sections after 48 h treatment with control (DMSO 0.1%), cisplatin, and EPZ6438. Immunohistochemical staining of ( A ) C33a and ( B ) CaSki for expression of EZH2, p53, ZO-1, β-catenin, and HPV16 E6 from the CAM tissue model (n = 1). Scale = 50 µm. Chorionic (CE) and allantoic (AE) epithelial layers with sub-epithelial capillary network (SEC), tumour cells (TC), mesoderm (M), and blood vessels (BV) are displayed.

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: Immunohistochemical staining, Staining, Chick Chorioallantoic Membrane Assay, Control, Expressing

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Expression of the EGFR/HER-receptor family in human lung cancer and a cervix cancer cell-line. RT-PCR was performed with 12 non-small cell lung cancer (NSCLC) cell lines and one cervical cancer cell line (C33A) to evaluate the expression of EGFR/HER2, HER3, and HER4-receptor mRNA. β-Actin was used as a loading control. Primers used are shown in , and experimental conditions are as described in Materials and Methods. This experiment is representative of 2 others. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Protein expression of HER4, GRPR, and NRG1 in 9 NSCLC cell lines as well as the cervix cancer cell line C33A. ( A ) Representative blot of HER4, GRPR, NRG1, and Tubulin. ( B ) Relative protein expression of HER4, GRPR, and NRG1. The cell lysates were subjected to Western blotting and analyzed using anti-HER4, anti-GRPR, anti-NRG1, and, as a loading control, anti-tubulin. Bands were visualized using chemiluminescence. Results are expressed as the ratio of the protein expression compared to tubulin. This experiment is representative of 2 others. Abbreviations: GRPR, Gastrin-Releasing Peptide Receptor; NRG1, Neuregulin-1. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Western Blot, Control

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Expression of the Neuregulin family in human NSCLC cell lines, one cervical cancer cell line, one prostate cancer cell line, and one breast cancer cell line. PCR was performed with 9 human lung cancer cell lines, one cervical cancer cell line (C33A), and one prostate (Prostate-Z) and breast cancer cell line (MCF-7) to evaluate the expression of Neuregulin mRNA. β-Actin was used as a loading control. Primers used are shown in , and experimental conditions are as described in Materials and Methods. This experiment is representative of 2 others. Abbreviation: NRG, Neuregulin. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Control, Western Blot

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Expression of HER4 Isoforms in human NSCL cancer cell lines. RT-PCR was performed with 8 human lung cancer cell lines, one cervix cancer cell line (C33A), brain and cerebellum to evaluate the expression of HER4 Isoforms (JM-a, JM-b, CYT1, and CYT2) mRNA. β-Actin was used as a loading control. Primers used are shown in , and experimental conditions are as described in Materials and Methods. This experiment is representative of 2 others. Abbreviations: CYT, cytosolic C-terminus; JM, extracellular juxtamembrane region. JM-a, JM-b, CYT1, and CYT2 are HER4 splice variants. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot

Journal: Biomolecules and Biomedicine

Article Title: Role of Fascin-1 in cervical cancer metastasis via Wnt/β-catenin pathway activation

doi: 10.17305/bb.2025.12114

Figure Lengend Snippet: Impact of Fascin-1 on in vitro growth of cervical cancer cells. (A) Assessment of Fascin-1 levels in Hela and C33A cells through Western blot, post-infection with lentiviruses carrying either FSCN1 -targeting shRNAs or control shRNAs; (B) RT-PCR analysis of FSCN1 mRNA levels when knockdown FASCIN; (C) Evaluation of cell proliferation in Hela and C33A cells with FSCN1 or control shRNA expression, using MTT assays; (D) Growth of Hela and C33A cells expressing respective shRNAs, cultured for 10 days and stained with crystal violet; colony counts were recorded; (E) Western blot analysis of cell lysates from Hela and C33A cells stably expressing FLAG- FSCN1 empty vector; (F) RT-PCR analysis of FSCN1 mRNA levels when overexpressed FSCN1 ; (G) Proliferation analysis via MTT assays in cells with either control or FSCN1 overexpression; (H) Analysis of colony formation in cells with indicated treatments; (I) Cell cycle experiments in indicated cells. Data for – and – are presented as mean ± SD, n ═ 3; *** P < 0.001, ** P < 0.01, * P < 0.05 (Student’s t -test). shRNA: Short hairpin RNA.

Article Snippet: The Hela and C33A human cervical cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in RPMI 1640 and MEM media, respectively, both supplied by Meilunbio (Dalian, China).

Techniques: In Vitro, Western Blot, Infection, Control, Reverse Transcription Polymerase Chain Reaction, Knockdown, shRNA, Expressing, Cell Culture, Staining, Stable Transfection, Plasmid Preparation, Over Expression

Journal: Biomolecules and Biomedicine

Article Title: Role of Fascin-1 in cervical cancer metastasis via Wnt/β-catenin pathway activation

doi: 10.17305/bb.2025.12114

Figure Lengend Snippet: Enhancement of cervical cell migration by Fascin-1 in vitro . (A and B) Migration and invasion capacities of Hela and C33A cells, with either FSCN1 shRNAs or control shRNAs, were assessed through respective assays; (C and D) Cells overexpressing either empty vectors or FLAG-tagged FSCN1 were analyzed for migration and invasion abilities. Scale bars represent 100 µm. Statistical data are presented as mean ± SD ( n ═ 3); *** P < 0.001, ** P < 0.01, * P < 0.05 (Student’s t -test).

Article Snippet: The Hela and C33A human cervical cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in RPMI 1640 and MEM media, respectively, both supplied by Meilunbio (Dalian, China).

Techniques: Migration, In Vitro, Control

Journal: Biomolecules and Biomedicine

Article Title: Role of Fascin-1 in cervical cancer metastasis via Wnt/β-catenin pathway activation

doi: 10.17305/bb.2025.12114

Figure Lengend Snippet: Downregulation of Fascin-1 impairs Wnt/β-catenin signaling. (A) GSEA enrichment plot of Wnt signaling pathway using RNA-seq data generated from Hela cells; (B) Heatmap of genes involved in Wnt signaling pathway; (C) mRNA was isolated from Hela and C33A cells expressing specific shRNAs, and quantitative real-time RT-PCR assays were conducted to measure expression levels; (D) RT-PCR analysis of FSCN1 , CTNNB1 and c-Myc mRNA levels in the indicated cells; (E and F) Protein lysates from indicated Hela and C33A cells, were analyzed by Western blot using relevant antibodies; (G) pTop-Flash/pFop-Flash reporter vectors were used to evaluate the transcriptional activity of the Wnt/β-catenin signaling pathway. Each bar in the graph indicates the average ± SD from three independent experiments, *** P < 0.001 compared to control (Student’s t -test). GSEA: Gene set enrichment analysis.

Article Snippet: The Hela and C33A human cervical cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in RPMI 1640 and MEM media, respectively, both supplied by Meilunbio (Dalian, China).

Techniques: RNA Sequencing, Generated, Isolation, Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Western Blot, Activity Assay, Control